Review



grb7 (n-20) n-terminal grb7, rabbit polyclonal sc-607 antibody  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology grb7 (n-20) n-terminal grb7, rabbit polyclonal sc-607 antibody
    <t>Grb7</t> expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb14 in Fig. ). A Whole wild type embryo with expression highlighted in dermis (d), gut (g), inner ear (i), liver (li), lung (lu), nasal epithelium (ne), pancreas (p), pituitary (pi), ribs (r, ossified cartilage), stomach (s), salivary gland (sg) and tooth primordia (tp); B Expression in submandibular gland and tooth primordia; C Expression in kidney (ki) and adrenal gland (ad); D Expression in lung and liver, but not heart (h); E Expression in epithelial lining of mid- and hind-gut; F Endocrine pancreas. Brown staining is indicative of Grb7 expression, magnifications as indicated
    Grb7 (N 20) N Terminal Grb7, Rabbit Polyclonal Sc 607 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grb7 (n-20) n-terminal grb7, rabbit polyclonal sc-607 antibody/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    grb7 (n-20) n-terminal grb7, rabbit polyclonal sc-607 antibody - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism"

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    Journal: BMC Biology

    doi: 10.1186/s12915-024-02018-5

    Grb7 expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb14 in Fig. ). A Whole wild type embryo with expression highlighted in dermis (d), gut (g), inner ear (i), liver (li), lung (lu), nasal epithelium (ne), pancreas (p), pituitary (pi), ribs (r, ossified cartilage), stomach (s), salivary gland (sg) and tooth primordia (tp); B Expression in submandibular gland and tooth primordia; C Expression in kidney (ki) and adrenal gland (ad); D Expression in lung and liver, but not heart (h); E Expression in epithelial lining of mid- and hind-gut; F Endocrine pancreas. Brown staining is indicative of Grb7 expression, magnifications as indicated
    Figure Legend Snippet: Grb7 expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb14 in Fig. ). A Whole wild type embryo with expression highlighted in dermis (d), gut (g), inner ear (i), liver (li), lung (lu), nasal epithelium (ne), pancreas (p), pituitary (pi), ribs (r, ossified cartilage), stomach (s), salivary gland (sg) and tooth primordia (tp); B Expression in submandibular gland and tooth primordia; C Expression in kidney (ki) and adrenal gland (ad); D Expression in lung and liver, but not heart (h); E Expression in epithelial lining of mid- and hind-gut; F Endocrine pancreas. Brown staining is indicative of Grb7 expression, magnifications as indicated

    Techniques Used: Expressing, Immunohistochemistry, Staining

    Grb14 expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb7 in Fig. ). A Whole wild type embryo with expression highlighted in cardiac muscle (c), choroid plexus (cp), dermis (d), diaphragm (di), gut (g), lungs (lu), liver (li), ribs (r, ossifying cartilage), stomach (s), tooth primordia (tp) and tongue (t); B Expression in choroid plexus ; C Expression in ossifying cartilage (ca) of vertebrae; D Expression in lung, ossifying cartilage of ribs and intercostal muscle (ic); E Expression in pancreas (p). Brown staining is indicative of Grb14 expression, magnifications as indicated
    Figure Legend Snippet: Grb14 expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb7 in Fig. ). A Whole wild type embryo with expression highlighted in cardiac muscle (c), choroid plexus (cp), dermis (d), diaphragm (di), gut (g), lungs (lu), liver (li), ribs (r, ossifying cartilage), stomach (s), tooth primordia (tp) and tongue (t); B Expression in choroid plexus ; C Expression in ossifying cartilage (ca) of vertebrae; D Expression in lung, ossifying cartilage of ribs and intercostal muscle (ic); E Expression in pancreas (p). Brown staining is indicative of Grb14 expression, magnifications as indicated

    Techniques Used: Expressing, Immunohistochemistry, Staining

    Expression of Grb7 family members in PN21 mouse pancreas visualised by immunohistochemistry on paraffin sections. Antibodies specific for ( A ) Grb7; B Grb10; and C Grb14; were used to detect distinct proteins of the Grb7 family. Brown staining is indicative of protein expression. Arrows indicate endocrine area (pancreatic islets); magnification 100x
    Figure Legend Snippet: Expression of Grb7 family members in PN21 mouse pancreas visualised by immunohistochemistry on paraffin sections. Antibodies specific for ( A ) Grb7; B Grb10; and C Grb14; were used to detect distinct proteins of the Grb7 family. Brown staining is indicative of protein expression. Arrows indicate endocrine area (pancreatic islets); magnification 100x

    Techniques Used: Expressing, Immunohistochemistry, Staining

    Genetic crosses used in the study, showing parent and offspring genotypes with their expected ratios. Crosses between (A) Grb10 +/p : Grb14 +/- double heterozygous females and Grb10 +/p : Grb14 +/- double heterozygous males, producing offspring of twelve genotypes, and (B) Grb7 +/- : Grb10 +/p double heterozygous females and Grb7 +/- heterozygous males, producing offspring of six genotypes, each in the indicated, expected Mendelian ratios. For ANOVA statistical analysis these six or twelve genotypes were used to form four groups, as indicated, since no difference was anticipated between animals differing only in their Grb14 +/- and Grb14 +/+ or Grb7 +/- and Grb7 +/+ allelic status. Similarly, due to the imprinted expression of Grb10 , offspring inheriting a mutant copy of the paternal Grb10 allele ( Grb10 +/p ), having no growth phenotype, were not expected to differ from Grb10 wild type ( Grb10 +/+ ) offspring, while double knockout (DKO) offspring inheriting mutations of both parental alleles ( Grb10 m/p ) were expected to be indistinguishable from those inheriting a mutant copy of the normally active maternal Grb10 allele ( Grb10 m/+ )
    Figure Legend Snippet: Genetic crosses used in the study, showing parent and offspring genotypes with their expected ratios. Crosses between (A) Grb10 +/p : Grb14 +/- double heterozygous females and Grb10 +/p : Grb14 +/- double heterozygous males, producing offspring of twelve genotypes, and (B) Grb7 +/- : Grb10 +/p double heterozygous females and Grb7 +/- heterozygous males, producing offspring of six genotypes, each in the indicated, expected Mendelian ratios. For ANOVA statistical analysis these six or twelve genotypes were used to form four groups, as indicated, since no difference was anticipated between animals differing only in their Grb14 +/- and Grb14 +/+ or Grb7 +/- and Grb7 +/+ allelic status. Similarly, due to the imprinted expression of Grb10 , offspring inheriting a mutant copy of the paternal Grb10 allele ( Grb10 +/p ), having no growth phenotype, were not expected to differ from Grb10 wild type ( Grb10 +/+ ) offspring, while double knockout (DKO) offspring inheriting mutations of both parental alleles ( Grb10 m/p ) were expected to be indistinguishable from those inheriting a mutant copy of the normally active maternal Grb10 allele ( Grb10 m/+ )

    Techniques Used: Expressing, Mutagenesis, Double Knockout

    Summary of PN1 body and organ weight data for progeny of crosses between (A) the Grb10 KO and Grb14 KO strains, and (B) the Grb7 KO and Grb10 KO strains. Mean weights are shown for each genotype together with changes relative to wild type (%WT) for each mutant genotype
    Figure Legend Snippet: Summary of PN1 body and organ weight data for progeny of crosses between (A) the Grb10 KO and Grb14 KO strains, and (B) the Grb7 KO and Grb10 KO strains. Mean weights are shown for each genotype together with changes relative to wild type (%WT) for each mutant genotype

    Techniques Used: Mutagenesis

    Generation of a Grb7 KO mouse strain. A null mutation was designed, lacking all the protein coding sequence. A The wild type Grb7 locus (top), showing exons (filled boxes numbered 1–15), translational start (open triangle) and stop (filled triangle) codons, plus selected restriction enzyme sites. Note that the translational start (ATG) codon lies within a Nco I restriction site (CCATGG) used as part of the cloning strategy. Regions used to direct homologous recombination in ES cells (5’ arm and 3’ arm) and also probes used to confirm correct targeting are indicated to either side of the coding exons. The homologous arms were cloned into the pPNT vector to form the pPNT- Grb7 targeting construct (middle), in which the coding exons have been replaced with a neomycin ( neo ) resistance gene cassette for positive selection of ES cells stably incorporating the construct. The targeting construct also includes a herpes simplex virus thymidine kinase ( hsv-tk ) gene, located outside the homologous regions that is typically retained at sites of random integration, and was used to enrich for targeting events by negative selection. Consequently, the correctly targeted allele (bottom) retains the neo but not the hsv-tk gene. B Southern blot analysis of Hind III digested DNA from wild type ES cells (ES) or primary mouse embryonic fibroblasts (MEF) alongside three successfully targeted ES cell clones (2E5, 4B5 and 5D1). In the targeted (TG) allele, loss of the sequence between the homologous arms alters the distance between restriction enzyme sites, compared with the wild type (WT) allele. Consequently, in the wild type allele Probe A recognises a 5′ 8.7 kb fragment and Probe B a 3′ 10.8 kb fragment, whereas both probes detect a15.3 kb fragment for the targeted allele. C Western blot analysis of protein extracts from adult kidney and liver derived from Grb7 wild type (+ / +) and Grb7 KO (-/-) homozygous mice following establishment of a true breeding line. The blot was probed with an antibody specific for Grb7, that readily detects a species of approximately the correct size (65 kDa) in wild type but not Grb7 KO samples, whereas an antibody specific for α-tubulin (50 kDa) was detected equally in both
    Figure Legend Snippet: Generation of a Grb7 KO mouse strain. A null mutation was designed, lacking all the protein coding sequence. A The wild type Grb7 locus (top), showing exons (filled boxes numbered 1–15), translational start (open triangle) and stop (filled triangle) codons, plus selected restriction enzyme sites. Note that the translational start (ATG) codon lies within a Nco I restriction site (CCATGG) used as part of the cloning strategy. Regions used to direct homologous recombination in ES cells (5’ arm and 3’ arm) and also probes used to confirm correct targeting are indicated to either side of the coding exons. The homologous arms were cloned into the pPNT vector to form the pPNT- Grb7 targeting construct (middle), in which the coding exons have been replaced with a neomycin ( neo ) resistance gene cassette for positive selection of ES cells stably incorporating the construct. The targeting construct also includes a herpes simplex virus thymidine kinase ( hsv-tk ) gene, located outside the homologous regions that is typically retained at sites of random integration, and was used to enrich for targeting events by negative selection. Consequently, the correctly targeted allele (bottom) retains the neo but not the hsv-tk gene. B Southern blot analysis of Hind III digested DNA from wild type ES cells (ES) or primary mouse embryonic fibroblasts (MEF) alongside three successfully targeted ES cell clones (2E5, 4B5 and 5D1). In the targeted (TG) allele, loss of the sequence between the homologous arms alters the distance between restriction enzyme sites, compared with the wild type (WT) allele. Consequently, in the wild type allele Probe A recognises a 5′ 8.7 kb fragment and Probe B a 3′ 10.8 kb fragment, whereas both probes detect a15.3 kb fragment for the targeted allele. C Western blot analysis of protein extracts from adult kidney and liver derived from Grb7 wild type (+ / +) and Grb7 KO (-/-) homozygous mice following establishment of a true breeding line. The blot was probed with an antibody specific for Grb7, that readily detects a species of approximately the correct size (65 kDa) in wild type but not Grb7 KO samples, whereas an antibody specific for α-tubulin (50 kDa) was detected equally in both

    Techniques Used: Mutagenesis, Sequencing, Cloning, Homologous Recombination, Clone Assay, Plasmid Preparation, Construct, Selection, Stable Transfection, Virus, Southern Blot, Western Blot, Derivative Assay

    Weights and blood glucose levels in PN1 progeny from Grb7 KO x Grb10 KO crosses. Weights of whole body and selected dissected organs, with blood glucose levels were collected at PN1 from progeny of crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockout (DKO). For each of the four offspring genotype groups, data are shown for, A Body weight; and B Blood glucose concentration ([glu]). In addition, actual weights of ( C ) Brain; D Liver; E Lungs; F Heart and G Kidneys are shown above the relative weights of the same organs, expressed as a percentage of body mass ( H – L ). Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for body and brain, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; kidneys and heart, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 48, Grb7 : Grb10 DKO N = 15, liver, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 14; lungs, WT N = 41, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; glucose levels, WT N = 24, Grb7 KO N = 7, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 10. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001
    Figure Legend Snippet: Weights and blood glucose levels in PN1 progeny from Grb7 KO x Grb10 KO crosses. Weights of whole body and selected dissected organs, with blood glucose levels were collected at PN1 from progeny of crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockout (DKO). For each of the four offspring genotype groups, data are shown for, A Body weight; and B Blood glucose concentration ([glu]). In addition, actual weights of ( C ) Brain; D Liver; E Lungs; F Heart and G Kidneys are shown above the relative weights of the same organs, expressed as a percentage of body mass ( H – L ). Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for body and brain, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; kidneys and heart, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 48, Grb7 : Grb10 DKO N = 15, liver, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 14; lungs, WT N = 41, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; glucose levels, WT N = 24, Grb7 KO N = 7, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 10. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001

    Techniques Used: Double Knockout, Concentration Assay

    Weight analysis of e17.5 conceptuses from crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockouts (DKO). Weights are shown for the four offspring genotype groups for ( A ) Embryo; and B Placenta. C These values have been used to calculate the embryo to placenta weight ratio as a measure of placental efficiency. Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for embryo WT N = 24, Grb7 KO N = 5, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 6, and for placenta and embryo:placenta ratio WT N = 23, Grb7 KO N = 5, Grb10 KO N = 27, Grb7:Grb10 DKO N = 6. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001
    Figure Legend Snippet: Weight analysis of e17.5 conceptuses from crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockouts (DKO). Weights are shown for the four offspring genotype groups for ( A ) Embryo; and B Placenta. C These values have been used to calculate the embryo to placenta weight ratio as a measure of placental efficiency. Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for embryo WT N = 24, Grb7 KO N = 5, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 6, and for placenta and embryo:placenta ratio WT N = 23, Grb7 KO N = 5, Grb10 KO N = 27, Grb7:Grb10 DKO N = 6. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001

    Techniques Used:

    Body composition analysis of adult Grb7 KO mice compared to wild type (WT) mice. Dual X-ray absorptiometry (DXA) analysis of 15 week old males ( A - F ) and females ( G - L ), showing estimates of total body weight ( A , G ), lean body content ( B , H ), fat body content ( C , I ), fat as a proportion of body weight ( D , J ), bone mineral content ( E , K ), and bone mineral density ( F , L ). For the same animals, physical weights were then obtained for the body and selected tissues and organs for both males ( M - R ) and females ( S - X ). Physical weight data are shown for total body ( M , S ), masseter muscle ( N , T ); gonadal WAT ( O , U ) and renal WAT ( Q , W ), along with weights as a proportion of body weight for gonadal WAT ( P , V ) and renal WAT ( R , X ). Graphs show means and SEM, and differences between the means were evaluated using a two-sided Student’s t-test. Sample sizes for DXA measurements were, for males WT N = 9, Grb7 KO N = 10 and females WT N = 5, Grb7 KO N = 3. Sample sizes for physical weight data were, for males WT N = 12, Grb7 KO N = 13, and for females WT N = 9, Grb7 KO N = 7. Asterisks indicate p- values, * p < 0.05
    Figure Legend Snippet: Body composition analysis of adult Grb7 KO mice compared to wild type (WT) mice. Dual X-ray absorptiometry (DXA) analysis of 15 week old males ( A - F ) and females ( G - L ), showing estimates of total body weight ( A , G ), lean body content ( B , H ), fat body content ( C , I ), fat as a proportion of body weight ( D , J ), bone mineral content ( E , K ), and bone mineral density ( F , L ). For the same animals, physical weights were then obtained for the body and selected tissues and organs for both males ( M - R ) and females ( S - X ). Physical weight data are shown for total body ( M , S ), masseter muscle ( N , T ); gonadal WAT ( O , U ) and renal WAT ( Q , W ), along with weights as a proportion of body weight for gonadal WAT ( P , V ) and renal WAT ( R , X ). Graphs show means and SEM, and differences between the means were evaluated using a two-sided Student’s t-test. Sample sizes for DXA measurements were, for males WT N = 9, Grb7 KO N = 10 and females WT N = 5, Grb7 KO N = 3. Sample sizes for physical weight data were, for males WT N = 12, Grb7 KO N = 13, and for females WT N = 9, Grb7 KO N = 7. Asterisks indicate p- values, * p < 0.05

    Techniques Used:

    Glucose homeostasis in adult Grb7 KO mice compared to wild type. Circulating glucose levels were compared for both male ( A - C ) and female ( D - F ) wild type (WT) and Grb7 KO mice, aged 14 weeks, in the fasted ( A , D ) and fed ( B , E ) states. The same animals were also tested for the ability to clear a glucose load after a fasting period in a standard glucose tolerance test ( C , F ). Glucose levels were measured at intervals over a time-course of 120 min with areas under the curve (AUC) measured in each case for statistical comparison. Graphs show means and SEM, and differences between the means were evaluated using a two-sided Student’s t-test. Sample sizes for male fasted and fed glucose levels were WT N = 12, Grb7 KO N = 13, for female fasted glucose WT N = 9, Grb7 KO N = 7, and fed glucose WT N = 9, Grb7 KO N = 6. Sample sizes for glucose tolerance tests were, for males WT N = 10, Grb7 KO N = 11, and for females WT N = 9, Grb7 KO N = 7. Asterisks indicate p- values, * p < 0.05, ** p < 0.01
    Figure Legend Snippet: Glucose homeostasis in adult Grb7 KO mice compared to wild type. Circulating glucose levels were compared for both male ( A - C ) and female ( D - F ) wild type (WT) and Grb7 KO mice, aged 14 weeks, in the fasted ( A , D ) and fed ( B , E ) states. The same animals were also tested for the ability to clear a glucose load after a fasting period in a standard glucose tolerance test ( C , F ). Glucose levels were measured at intervals over a time-course of 120 min with areas under the curve (AUC) measured in each case for statistical comparison. Graphs show means and SEM, and differences between the means were evaluated using a two-sided Student’s t-test. Sample sizes for male fasted and fed glucose levels were WT N = 12, Grb7 KO N = 13, for female fasted glucose WT N = 9, Grb7 KO N = 7, and fed glucose WT N = 9, Grb7 KO N = 6. Sample sizes for glucose tolerance tests were, for males WT N = 10, Grb7 KO N = 11, and for females WT N = 9, Grb7 KO N = 7. Asterisks indicate p- values, * p < 0.05, ** p < 0.01

    Techniques Used: Comparison

    Grb7, Grb10 and Grb14 distribution in the developing mouse embryo
    Figure Legend Snippet: Grb7, Grb10 and Grb14 distribution in the developing mouse embryo

    Techniques Used:



    Similar Products

    grb7 (n-20) n-terminal grb7, rabbit polyclonal sc-607 antibody  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology grb7 (n-20) n-terminal grb7, rabbit polyclonal sc-607 antibody
    <t>Grb7</t> expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb14 in Fig. ). A Whole wild type embryo with expression highlighted in dermis (d), gut (g), inner ear (i), liver (li), lung (lu), nasal epithelium (ne), pancreas (p), pituitary (pi), ribs (r, ossified cartilage), stomach (s), salivary gland (sg) and tooth primordia (tp); B Expression in submandibular gland and tooth primordia; C Expression in kidney (ki) and adrenal gland (ad); D Expression in lung and liver, but not heart (h); E Expression in epithelial lining of mid- and hind-gut; F Endocrine pancreas. Brown staining is indicative of Grb7 expression, magnifications as indicated
    Grb7 (N 20) N Terminal Grb7, Rabbit Polyclonal Sc 607 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grb7 (n-20) n-terminal grb7, rabbit polyclonal sc-607 antibody/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    grb7 (n-20) n-terminal grb7, rabbit polyclonal sc-607 antibody - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    rabbit polyclonal anti grb7  (Proteintech)
    93
    Proteintech rabbit polyclonal anti grb7
    <t>Grb7</t> expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb14 in Fig. ). A Whole wild type embryo with expression highlighted in dermis (d), gut (g), inner ear (i), liver (li), lung (lu), nasal epithelium (ne), pancreas (p), pituitary (pi), ribs (r, ossified cartilage), stomach (s), salivary gland (sg) and tooth primordia (tp); B Expression in submandibular gland and tooth primordia; C Expression in kidney (ki) and adrenal gland (ad); D Expression in lung and liver, but not heart (h); E Expression in epithelial lining of mid- and hind-gut; F Endocrine pancreas. Brown staining is indicative of Grb7 expression, magnifications as indicated
    Rabbit Polyclonal Anti Grb7, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti grb7/product/Proteintech
    Average 93 stars, based on 1 article reviews
    rabbit polyclonal anti grb7 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    rabbit polyclonal anti grb7  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology rabbit polyclonal anti grb7
    <t>Grb7</t> expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb14 in Fig. ). A Whole wild type embryo with expression highlighted in dermis (d), gut (g), inner ear (i), liver (li), lung (lu), nasal epithelium (ne), pancreas (p), pituitary (pi), ribs (r, ossified cartilage), stomach (s), salivary gland (sg) and tooth primordia (tp); B Expression in submandibular gland and tooth primordia; C Expression in kidney (ki) and adrenal gland (ad); D Expression in lung and liver, but not heart (h); E Expression in epithelial lining of mid- and hind-gut; F Endocrine pancreas. Brown staining is indicative of Grb7 expression, magnifications as indicated
    Rabbit Polyclonal Anti Grb7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti grb7/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    rabbit polyclonal anti grb7 - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    grb7 polyclonal antibody  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology grb7 polyclonal antibody
    <t>Grb7</t> expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb14 in Fig. ). A Whole wild type embryo with expression highlighted in dermis (d), gut (g), inner ear (i), liver (li), lung (lu), nasal epithelium (ne), pancreas (p), pituitary (pi), ribs (r, ossified cartilage), stomach (s), salivary gland (sg) and tooth primordia (tp); B Expression in submandibular gland and tooth primordia; C Expression in kidney (ki) and adrenal gland (ad); D Expression in lung and liver, but not heart (h); E Expression in epithelial lining of mid- and hind-gut; F Endocrine pancreas. Brown staining is indicative of Grb7 expression, magnifications as indicated
    Grb7 Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grb7 polyclonal antibody/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    grb7 polyclonal antibody - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    polyclonal anti grb7 antibody  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology polyclonal anti grb7 antibody
    The expression of miR-193a-3p is downregulated and inversely correlates with <t>GRB7</t> expression in ovarian cancers. (A) Comparison of GRB7 expression in human ovarian cancer with other tumor types using the cBioPortal database. The dots represent clinical cases, and the line in the upper and lower box plot represents the upper and lower quartile of the relative mRNA levels of all samples. (B) The Venn diagram shows miR-193a-3p and miR-193b-3p commonly predicted by miRDB, TargetScan, and Miranda. (C) A schematic diagram showing the same binding site of miR-193a-3p and miR-193b-3p at position 332-338 of human GRB7 3' UTR. (D) Western blot analysis of GRB7 expression in ovarian cancer cell lines and HOSEs. (E) qPCR analysis of miR-193a-3p ( upper ) and miR-193b-3p ( lower ) expression in ovarian cancer cell lines. SNORD48 was used as an internal control. (F) Western blot analysis showing that transient transfection of pmR-ZsGreen1-miR-193a-3p or pmR-ZsGreen1-miR-193b-3p reduced the expression of GRB7 in SKOV3 or OVCA433 cells in a dose-dependent manner. (G) A schematic diagram showing the putative miR-193a-3p binding site in wild-type and mutant GRB7 3'UTR ( Upper ). Luciferase reporter assay showing the relative luciferase activity of wild-type or mutant GRB7 3'UTR targeted by a miR-193a-3p using pmR-ZsGreen1-miR-193a-3p plasmid in HEK293 cells ( Lower ).
    Polyclonal Anti Grb7 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti grb7 antibody/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    polyclonal anti grb7 antibody - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    anti grb7 n 20 rabbit polyclonal antibody  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology anti grb7 n 20 rabbit polyclonal antibody
    The expression of miR-193a-3p is downregulated and inversely correlates with <t>GRB7</t> expression in ovarian cancers. (A) Comparison of GRB7 expression in human ovarian cancer with other tumor types using the cBioPortal database. The dots represent clinical cases, and the line in the upper and lower box plot represents the upper and lower quartile of the relative mRNA levels of all samples. (B) The Venn diagram shows miR-193a-3p and miR-193b-3p commonly predicted by miRDB, TargetScan, and Miranda. (C) A schematic diagram showing the same binding site of miR-193a-3p and miR-193b-3p at position 332-338 of human GRB7 3' UTR. (D) Western blot analysis of GRB7 expression in ovarian cancer cell lines and HOSEs. (E) qPCR analysis of miR-193a-3p ( upper ) and miR-193b-3p ( lower ) expression in ovarian cancer cell lines. SNORD48 was used as an internal control. (F) Western blot analysis showing that transient transfection of pmR-ZsGreen1-miR-193a-3p or pmR-ZsGreen1-miR-193b-3p reduced the expression of GRB7 in SKOV3 or OVCA433 cells in a dose-dependent manner. (G) A schematic diagram showing the putative miR-193a-3p binding site in wild-type and mutant GRB7 3'UTR ( Upper ). Luciferase reporter assay showing the relative luciferase activity of wild-type or mutant GRB7 3'UTR targeted by a miR-193a-3p using pmR-ZsGreen1-miR-193a-3p plasmid in HEK293 cells ( Lower ).
    Anti Grb7 N 20 Rabbit Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti grb7 n 20 rabbit polyclonal antibody/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    anti grb7 n 20 rabbit polyclonal antibody - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    anti-grb7 (rabbit) polyclonal antibody (h-70)  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology anti-grb7 (rabbit) polyclonal antibody (h-70)
    The expression of miR-193a-3p is downregulated and inversely correlates with <t>GRB7</t> expression in ovarian cancers. (A) Comparison of GRB7 expression in human ovarian cancer with other tumor types using the cBioPortal database. The dots represent clinical cases, and the line in the upper and lower box plot represents the upper and lower quartile of the relative mRNA levels of all samples. (B) The Venn diagram shows miR-193a-3p and miR-193b-3p commonly predicted by miRDB, TargetScan, and Miranda. (C) A schematic diagram showing the same binding site of miR-193a-3p and miR-193b-3p at position 332-338 of human GRB7 3' UTR. (D) Western blot analysis of GRB7 expression in ovarian cancer cell lines and HOSEs. (E) qPCR analysis of miR-193a-3p ( upper ) and miR-193b-3p ( lower ) expression in ovarian cancer cell lines. SNORD48 was used as an internal control. (F) Western blot analysis showing that transient transfection of pmR-ZsGreen1-miR-193a-3p or pmR-ZsGreen1-miR-193b-3p reduced the expression of GRB7 in SKOV3 or OVCA433 cells in a dose-dependent manner. (G) A schematic diagram showing the putative miR-193a-3p binding site in wild-type and mutant GRB7 3'UTR ( Upper ). Luciferase reporter assay showing the relative luciferase activity of wild-type or mutant GRB7 3'UTR targeted by a miR-193a-3p using pmR-ZsGreen1-miR-193a-3p plasmid in HEK293 cells ( Lower ).
    Anti Grb7 (Rabbit) Polyclonal Antibody (H 70), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-grb7 (rabbit) polyclonal antibody (h-70)/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    anti-grb7 (rabbit) polyclonal antibody (h-70) - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    anti-grb7 (rabbit) polyclonal antibody (h-70) (product number sc-13954)  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology anti-grb7 (rabbit) polyclonal antibody (h-70) (product number sc-13954)
    The expression of miR-193a-3p is downregulated and inversely correlates with <t>GRB7</t> expression in ovarian cancers. (A) Comparison of GRB7 expression in human ovarian cancer with other tumor types using the cBioPortal database. The dots represent clinical cases, and the line in the upper and lower box plot represents the upper and lower quartile of the relative mRNA levels of all samples. (B) The Venn diagram shows miR-193a-3p and miR-193b-3p commonly predicted by miRDB, TargetScan, and Miranda. (C) A schematic diagram showing the same binding site of miR-193a-3p and miR-193b-3p at position 332-338 of human GRB7 3' UTR. (D) Western blot analysis of GRB7 expression in ovarian cancer cell lines and HOSEs. (E) qPCR analysis of miR-193a-3p ( upper ) and miR-193b-3p ( lower ) expression in ovarian cancer cell lines. SNORD48 was used as an internal control. (F) Western blot analysis showing that transient transfection of pmR-ZsGreen1-miR-193a-3p or pmR-ZsGreen1-miR-193b-3p reduced the expression of GRB7 in SKOV3 or OVCA433 cells in a dose-dependent manner. (G) A schematic diagram showing the putative miR-193a-3p binding site in wild-type and mutant GRB7 3'UTR ( Upper ). Luciferase reporter assay showing the relative luciferase activity of wild-type or mutant GRB7 3'UTR targeted by a miR-193a-3p using pmR-ZsGreen1-miR-193a-3p plasmid in HEK293 cells ( Lower ).
    Anti Grb7 (Rabbit) Polyclonal Antibody (H 70) (Product Number Sc 13954), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-grb7 (rabbit) polyclonal antibody (h-70) (product number sc-13954)/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    anti-grb7 (rabbit) polyclonal antibody (h-70) (product number sc-13954) - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    polyclonal anti grb7 antibody  (Boster Bio)
    90
    Boster Bio polyclonal anti grb7 antibody
    The expression of miR-193a-3p is downregulated and inversely correlates with <t>GRB7</t> expression in ovarian cancers. (A) Comparison of GRB7 expression in human ovarian cancer with other tumor types using the cBioPortal database. The dots represent clinical cases, and the line in the upper and lower box plot represents the upper and lower quartile of the relative mRNA levels of all samples. (B) The Venn diagram shows miR-193a-3p and miR-193b-3p commonly predicted by miRDB, TargetScan, and Miranda. (C) A schematic diagram showing the same binding site of miR-193a-3p and miR-193b-3p at position 332-338 of human GRB7 3' UTR. (D) Western blot analysis of GRB7 expression in ovarian cancer cell lines and HOSEs. (E) qPCR analysis of miR-193a-3p ( upper ) and miR-193b-3p ( lower ) expression in ovarian cancer cell lines. SNORD48 was used as an internal control. (F) Western blot analysis showing that transient transfection of pmR-ZsGreen1-miR-193a-3p or pmR-ZsGreen1-miR-193b-3p reduced the expression of GRB7 in SKOV3 or OVCA433 cells in a dose-dependent manner. (G) A schematic diagram showing the putative miR-193a-3p binding site in wild-type and mutant GRB7 3'UTR ( Upper ). Luciferase reporter assay showing the relative luciferase activity of wild-type or mutant GRB7 3'UTR targeted by a miR-193a-3p using pmR-ZsGreen1-miR-193a-3p plasmid in HEK293 cells ( Lower ).
    Polyclonal Anti Grb7 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti grb7 antibody/product/Boster Bio
    Average 90 stars, based on 1 article reviews
    polyclonal anti grb7 antibody - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Grb7 expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb14 in Fig. ). A Whole wild type embryo with expression highlighted in dermis (d), gut (g), inner ear (i), liver (li), lung (lu), nasal epithelium (ne), pancreas (p), pituitary (pi), ribs (r, ossified cartilage), stomach (s), salivary gland (sg) and tooth primordia (tp); B Expression in submandibular gland and tooth primordia; C Expression in kidney (ki) and adrenal gland (ad); D Expression in lung and liver, but not heart (h); E Expression in epithelial lining of mid- and hind-gut; F Endocrine pancreas. Brown staining is indicative of Grb7 expression, magnifications as indicated

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Grb7 expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb14 in Fig. ). A Whole wild type embryo with expression highlighted in dermis (d), gut (g), inner ear (i), liver (li), lung (lu), nasal epithelium (ne), pancreas (p), pituitary (pi), ribs (r, ossified cartilage), stomach (s), salivary gland (sg) and tooth primordia (tp); B Expression in submandibular gland and tooth primordia; C Expression in kidney (ki) and adrenal gland (ad); D Expression in lung and liver, but not heart (h); E Expression in epithelial lining of mid- and hind-gut; F Endocrine pancreas. Brown staining is indicative of Grb7 expression, magnifications as indicated

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques: Expressing, Immunohistochemistry, Staining

    Grb14 expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb7 in Fig. ). A Whole wild type embryo with expression highlighted in cardiac muscle (c), choroid plexus (cp), dermis (d), diaphragm (di), gut (g), lungs (lu), liver (li), ribs (r, ossifying cartilage), stomach (s), tooth primordia (tp) and tongue (t); B Expression in choroid plexus ; C Expression in ossifying cartilage (ca) of vertebrae; D Expression in lung, ossifying cartilage of ribs and intercostal muscle (ic); E Expression in pancreas (p). Brown staining is indicative of Grb14 expression, magnifications as indicated

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Grb14 expression in the mouse embryo visualised by immunohistochemistry on wild type e14.5 paraffin sections. Representative mid-sagittal sections were chosen to display a wide range of tissues (sections adjacent to those used to stain for Grb7 in Fig. ). A Whole wild type embryo with expression highlighted in cardiac muscle (c), choroid plexus (cp), dermis (d), diaphragm (di), gut (g), lungs (lu), liver (li), ribs (r, ossifying cartilage), stomach (s), tooth primordia (tp) and tongue (t); B Expression in choroid plexus ; C Expression in ossifying cartilage (ca) of vertebrae; D Expression in lung, ossifying cartilage of ribs and intercostal muscle (ic); E Expression in pancreas (p). Brown staining is indicative of Grb14 expression, magnifications as indicated

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques: Expressing, Immunohistochemistry, Staining

    Expression of Grb7 family members in PN21 mouse pancreas visualised by immunohistochemistry on paraffin sections. Antibodies specific for ( A ) Grb7; B Grb10; and C Grb14; were used to detect distinct proteins of the Grb7 family. Brown staining is indicative of protein expression. Arrows indicate endocrine area (pancreatic islets); magnification 100x

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Expression of Grb7 family members in PN21 mouse pancreas visualised by immunohistochemistry on paraffin sections. Antibodies specific for ( A ) Grb7; B Grb10; and C Grb14; were used to detect distinct proteins of the Grb7 family. Brown staining is indicative of protein expression. Arrows indicate endocrine area (pancreatic islets); magnification 100x

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques: Expressing, Immunohistochemistry, Staining

    Genetic crosses used in the study, showing parent and offspring genotypes with their expected ratios. Crosses between (A) Grb10 +/p : Grb14 +/- double heterozygous females and Grb10 +/p : Grb14 +/- double heterozygous males, producing offspring of twelve genotypes, and (B) Grb7 +/- : Grb10 +/p double heterozygous females and Grb7 +/- heterozygous males, producing offspring of six genotypes, each in the indicated, expected Mendelian ratios. For ANOVA statistical analysis these six or twelve genotypes were used to form four groups, as indicated, since no difference was anticipated between animals differing only in their Grb14 +/- and Grb14 +/+ or Grb7 +/- and Grb7 +/+ allelic status. Similarly, due to the imprinted expression of Grb10 , offspring inheriting a mutant copy of the paternal Grb10 allele ( Grb10 +/p ), having no growth phenotype, were not expected to differ from Grb10 wild type ( Grb10 +/+ ) offspring, while double knockout (DKO) offspring inheriting mutations of both parental alleles ( Grb10 m/p ) were expected to be indistinguishable from those inheriting a mutant copy of the normally active maternal Grb10 allele ( Grb10 m/+ )

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Genetic crosses used in the study, showing parent and offspring genotypes with their expected ratios. Crosses between (A) Grb10 +/p : Grb14 +/- double heterozygous females and Grb10 +/p : Grb14 +/- double heterozygous males, producing offspring of twelve genotypes, and (B) Grb7 +/- : Grb10 +/p double heterozygous females and Grb7 +/- heterozygous males, producing offspring of six genotypes, each in the indicated, expected Mendelian ratios. For ANOVA statistical analysis these six or twelve genotypes were used to form four groups, as indicated, since no difference was anticipated between animals differing only in their Grb14 +/- and Grb14 +/+ or Grb7 +/- and Grb7 +/+ allelic status. Similarly, due to the imprinted expression of Grb10 , offspring inheriting a mutant copy of the paternal Grb10 allele ( Grb10 +/p ), having no growth phenotype, were not expected to differ from Grb10 wild type ( Grb10 +/+ ) offspring, while double knockout (DKO) offspring inheriting mutations of both parental alleles ( Grb10 m/p ) were expected to be indistinguishable from those inheriting a mutant copy of the normally active maternal Grb10 allele ( Grb10 m/+ )

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques: Expressing, Mutagenesis, Double Knockout

    Summary of PN1 body and organ weight data for progeny of crosses between (A) the Grb10 KO and Grb14 KO strains, and (B) the Grb7 KO and Grb10 KO strains. Mean weights are shown for each genotype together with changes relative to wild type (%WT) for each mutant genotype

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Summary of PN1 body and organ weight data for progeny of crosses between (A) the Grb10 KO and Grb14 KO strains, and (B) the Grb7 KO and Grb10 KO strains. Mean weights are shown for each genotype together with changes relative to wild type (%WT) for each mutant genotype

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques: Mutagenesis

    Generation of a Grb7 KO mouse strain. A null mutation was designed, lacking all the protein coding sequence. A The wild type Grb7 locus (top), showing exons (filled boxes numbered 1–15), translational start (open triangle) and stop (filled triangle) codons, plus selected restriction enzyme sites. Note that the translational start (ATG) codon lies within a Nco I restriction site (CCATGG) used as part of the cloning strategy. Regions used to direct homologous recombination in ES cells (5’ arm and 3’ arm) and also probes used to confirm correct targeting are indicated to either side of the coding exons. The homologous arms were cloned into the pPNT vector to form the pPNT- Grb7 targeting construct (middle), in which the coding exons have been replaced with a neomycin ( neo ) resistance gene cassette for positive selection of ES cells stably incorporating the construct. The targeting construct also includes a herpes simplex virus thymidine kinase ( hsv-tk ) gene, located outside the homologous regions that is typically retained at sites of random integration, and was used to enrich for targeting events by negative selection. Consequently, the correctly targeted allele (bottom) retains the neo but not the hsv-tk gene. B Southern blot analysis of Hind III digested DNA from wild type ES cells (ES) or primary mouse embryonic fibroblasts (MEF) alongside three successfully targeted ES cell clones (2E5, 4B5 and 5D1). In the targeted (TG) allele, loss of the sequence between the homologous arms alters the distance between restriction enzyme sites, compared with the wild type (WT) allele. Consequently, in the wild type allele Probe A recognises a 5′ 8.7 kb fragment and Probe B a 3′ 10.8 kb fragment, whereas both probes detect a15.3 kb fragment for the targeted allele. C Western blot analysis of protein extracts from adult kidney and liver derived from Grb7 wild type (+ / +) and Grb7 KO (-/-) homozygous mice following establishment of a true breeding line. The blot was probed with an antibody specific for Grb7, that readily detects a species of approximately the correct size (65 kDa) in wild type but not Grb7 KO samples, whereas an antibody specific for α-tubulin (50 kDa) was detected equally in both

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Generation of a Grb7 KO mouse strain. A null mutation was designed, lacking all the protein coding sequence. A The wild type Grb7 locus (top), showing exons (filled boxes numbered 1–15), translational start (open triangle) and stop (filled triangle) codons, plus selected restriction enzyme sites. Note that the translational start (ATG) codon lies within a Nco I restriction site (CCATGG) used as part of the cloning strategy. Regions used to direct homologous recombination in ES cells (5’ arm and 3’ arm) and also probes used to confirm correct targeting are indicated to either side of the coding exons. The homologous arms were cloned into the pPNT vector to form the pPNT- Grb7 targeting construct (middle), in which the coding exons have been replaced with a neomycin ( neo ) resistance gene cassette for positive selection of ES cells stably incorporating the construct. The targeting construct also includes a herpes simplex virus thymidine kinase ( hsv-tk ) gene, located outside the homologous regions that is typically retained at sites of random integration, and was used to enrich for targeting events by negative selection. Consequently, the correctly targeted allele (bottom) retains the neo but not the hsv-tk gene. B Southern blot analysis of Hind III digested DNA from wild type ES cells (ES) or primary mouse embryonic fibroblasts (MEF) alongside three successfully targeted ES cell clones (2E5, 4B5 and 5D1). In the targeted (TG) allele, loss of the sequence between the homologous arms alters the distance between restriction enzyme sites, compared with the wild type (WT) allele. Consequently, in the wild type allele Probe A recognises a 5′ 8.7 kb fragment and Probe B a 3′ 10.8 kb fragment, whereas both probes detect a15.3 kb fragment for the targeted allele. C Western blot analysis of protein extracts from adult kidney and liver derived from Grb7 wild type (+ / +) and Grb7 KO (-/-) homozygous mice following establishment of a true breeding line. The blot was probed with an antibody specific for Grb7, that readily detects a species of approximately the correct size (65 kDa) in wild type but not Grb7 KO samples, whereas an antibody specific for α-tubulin (50 kDa) was detected equally in both

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques: Mutagenesis, Sequencing, Cloning, Homologous Recombination, Clone Assay, Plasmid Preparation, Construct, Selection, Stable Transfection, Virus, Southern Blot, Western Blot, Derivative Assay

    Weights and blood glucose levels in PN1 progeny from Grb7 KO x Grb10 KO crosses. Weights of whole body and selected dissected organs, with blood glucose levels were collected at PN1 from progeny of crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockout (DKO). For each of the four offspring genotype groups, data are shown for, A Body weight; and B Blood glucose concentration ([glu]). In addition, actual weights of ( C ) Brain; D Liver; E Lungs; F Heart and G Kidneys are shown above the relative weights of the same organs, expressed as a percentage of body mass ( H – L ). Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for body and brain, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; kidneys and heart, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 48, Grb7 : Grb10 DKO N = 15, liver, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 14; lungs, WT N = 41, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; glucose levels, WT N = 24, Grb7 KO N = 7, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 10. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Weights and blood glucose levels in PN1 progeny from Grb7 KO x Grb10 KO crosses. Weights of whole body and selected dissected organs, with blood glucose levels were collected at PN1 from progeny of crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockout (DKO). For each of the four offspring genotype groups, data are shown for, A Body weight; and B Blood glucose concentration ([glu]). In addition, actual weights of ( C ) Brain; D Liver; E Lungs; F Heart and G Kidneys are shown above the relative weights of the same organs, expressed as a percentage of body mass ( H – L ). Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for body and brain, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; kidneys and heart, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 48, Grb7 : Grb10 DKO N = 15, liver, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 14; lungs, WT N = 41, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; glucose levels, WT N = 24, Grb7 KO N = 7, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 10. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques: Double Knockout, Concentration Assay

    Weight analysis of e17.5 conceptuses from crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockouts (DKO). Weights are shown for the four offspring genotype groups for ( A ) Embryo; and B Placenta. C These values have been used to calculate the embryo to placenta weight ratio as a measure of placental efficiency. Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for embryo WT N = 24, Grb7 KO N = 5, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 6, and for placenta and embryo:placenta ratio WT N = 23, Grb7 KO N = 5, Grb10 KO N = 27, Grb7:Grb10 DKO N = 6. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Weight analysis of e17.5 conceptuses from crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockouts (DKO). Weights are shown for the four offspring genotype groups for ( A ) Embryo; and B Placenta. C These values have been used to calculate the embryo to placenta weight ratio as a measure of placental efficiency. Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for embryo WT N = 24, Grb7 KO N = 5, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 6, and for placenta and embryo:placenta ratio WT N = 23, Grb7 KO N = 5, Grb10 KO N = 27, Grb7:Grb10 DKO N = 6. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques:

    Body composition analysis of adult Grb7 KO mice compared to wild type (WT) mice. Dual X-ray absorptiometry (DXA) analysis of 15 week old males ( A - F ) and females ( G - L ), showing estimates of total body weight ( A , G ), lean body content ( B , H ), fat body content ( C , I ), fat as a proportion of body weight ( D , J ), bone mineral content ( E , K ), and bone mineral density ( F , L ). For the same animals, physical weights were then obtained for the body and selected tissues and organs for both males ( M - R ) and females ( S - X ). Physical weight data are shown for total body ( M , S ), masseter muscle ( N , T ); gonadal WAT ( O , U ) and renal WAT ( Q , W ), along with weights as a proportion of body weight for gonadal WAT ( P , V ) and renal WAT ( R , X ). Graphs show means and SEM, and differences between the means were evaluated using a two-sided Student’s t-test. Sample sizes for DXA measurements were, for males WT N = 9, Grb7 KO N = 10 and females WT N = 5, Grb7 KO N = 3. Sample sizes for physical weight data were, for males WT N = 12, Grb7 KO N = 13, and for females WT N = 9, Grb7 KO N = 7. Asterisks indicate p- values, * p < 0.05

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Body composition analysis of adult Grb7 KO mice compared to wild type (WT) mice. Dual X-ray absorptiometry (DXA) analysis of 15 week old males ( A - F ) and females ( G - L ), showing estimates of total body weight ( A , G ), lean body content ( B , H ), fat body content ( C , I ), fat as a proportion of body weight ( D , J ), bone mineral content ( E , K ), and bone mineral density ( F , L ). For the same animals, physical weights were then obtained for the body and selected tissues and organs for both males ( M - R ) and females ( S - X ). Physical weight data are shown for total body ( M , S ), masseter muscle ( N , T ); gonadal WAT ( O , U ) and renal WAT ( Q , W ), along with weights as a proportion of body weight for gonadal WAT ( P , V ) and renal WAT ( R , X ). Graphs show means and SEM, and differences between the means were evaluated using a two-sided Student’s t-test. Sample sizes for DXA measurements were, for males WT N = 9, Grb7 KO N = 10 and females WT N = 5, Grb7 KO N = 3. Sample sizes for physical weight data were, for males WT N = 12, Grb7 KO N = 13, and for females WT N = 9, Grb7 KO N = 7. Asterisks indicate p- values, * p < 0.05

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques:

    Glucose homeostasis in adult Grb7 KO mice compared to wild type. Circulating glucose levels were compared for both male ( A - C ) and female ( D - F ) wild type (WT) and Grb7 KO mice, aged 14 weeks, in the fasted ( A , D ) and fed ( B , E ) states. The same animals were also tested for the ability to clear a glucose load after a fasting period in a standard glucose tolerance test ( C , F ). Glucose levels were measured at intervals over a time-course of 120 min with areas under the curve (AUC) measured in each case for statistical comparison. Graphs show means and SEM, and differences between the means were evaluated using a two-sided Student’s t-test. Sample sizes for male fasted and fed glucose levels were WT N = 12, Grb7 KO N = 13, for female fasted glucose WT N = 9, Grb7 KO N = 7, and fed glucose WT N = 9, Grb7 KO N = 6. Sample sizes for glucose tolerance tests were, for males WT N = 10, Grb7 KO N = 11, and for females WT N = 9, Grb7 KO N = 7. Asterisks indicate p- values, * p < 0.05, ** p < 0.01

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Glucose homeostasis in adult Grb7 KO mice compared to wild type. Circulating glucose levels were compared for both male ( A - C ) and female ( D - F ) wild type (WT) and Grb7 KO mice, aged 14 weeks, in the fasted ( A , D ) and fed ( B , E ) states. The same animals were also tested for the ability to clear a glucose load after a fasting period in a standard glucose tolerance test ( C , F ). Glucose levels were measured at intervals over a time-course of 120 min with areas under the curve (AUC) measured in each case for statistical comparison. Graphs show means and SEM, and differences between the means were evaluated using a two-sided Student’s t-test. Sample sizes for male fasted and fed glucose levels were WT N = 12, Grb7 KO N = 13, for female fasted glucose WT N = 9, Grb7 KO N = 7, and fed glucose WT N = 9, Grb7 KO N = 6. Sample sizes for glucose tolerance tests were, for males WT N = 10, Grb7 KO N = 11, and for females WT N = 9, Grb7 KO N = 7. Asterisks indicate p- values, * p < 0.05, ** p < 0.01

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques: Comparison

    Grb7, Grb10 and Grb14 distribution in the developing mouse embryo

    Journal: BMC Biology

    Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

    doi: 10.1186/s12915-024-02018-5

    Figure Lengend Snippet: Grb7, Grb10 and Grb14 distribution in the developing mouse embryo

    Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

    Techniques:

    The expression of miR-193a-3p is downregulated and inversely correlates with GRB7 expression in ovarian cancers. (A) Comparison of GRB7 expression in human ovarian cancer with other tumor types using the cBioPortal database. The dots represent clinical cases, and the line in the upper and lower box plot represents the upper and lower quartile of the relative mRNA levels of all samples. (B) The Venn diagram shows miR-193a-3p and miR-193b-3p commonly predicted by miRDB, TargetScan, and Miranda. (C) A schematic diagram showing the same binding site of miR-193a-3p and miR-193b-3p at position 332-338 of human GRB7 3' UTR. (D) Western blot analysis of GRB7 expression in ovarian cancer cell lines and HOSEs. (E) qPCR analysis of miR-193a-3p ( upper ) and miR-193b-3p ( lower ) expression in ovarian cancer cell lines. SNORD48 was used as an internal control. (F) Western blot analysis showing that transient transfection of pmR-ZsGreen1-miR-193a-3p or pmR-ZsGreen1-miR-193b-3p reduced the expression of GRB7 in SKOV3 or OVCA433 cells in a dose-dependent manner. (G) A schematic diagram showing the putative miR-193a-3p binding site in wild-type and mutant GRB7 3'UTR ( Upper ). Luciferase reporter assay showing the relative luciferase activity of wild-type or mutant GRB7 3'UTR targeted by a miR-193a-3p using pmR-ZsGreen1-miR-193a-3p plasmid in HEK293 cells ( Lower ).

    Journal: Theranostics

    Article Title: Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways

    doi: 10.7150/thno.22377

    Figure Lengend Snippet: The expression of miR-193a-3p is downregulated and inversely correlates with GRB7 expression in ovarian cancers. (A) Comparison of GRB7 expression in human ovarian cancer with other tumor types using the cBioPortal database. The dots represent clinical cases, and the line in the upper and lower box plot represents the upper and lower quartile of the relative mRNA levels of all samples. (B) The Venn diagram shows miR-193a-3p and miR-193b-3p commonly predicted by miRDB, TargetScan, and Miranda. (C) A schematic diagram showing the same binding site of miR-193a-3p and miR-193b-3p at position 332-338 of human GRB7 3' UTR. (D) Western blot analysis of GRB7 expression in ovarian cancer cell lines and HOSEs. (E) qPCR analysis of miR-193a-3p ( upper ) and miR-193b-3p ( lower ) expression in ovarian cancer cell lines. SNORD48 was used as an internal control. (F) Western blot analysis showing that transient transfection of pmR-ZsGreen1-miR-193a-3p or pmR-ZsGreen1-miR-193b-3p reduced the expression of GRB7 in SKOV3 or OVCA433 cells in a dose-dependent manner. (G) A schematic diagram showing the putative miR-193a-3p binding site in wild-type and mutant GRB7 3'UTR ( Upper ). Luciferase reporter assay showing the relative luciferase activity of wild-type or mutant GRB7 3'UTR targeted by a miR-193a-3p using pmR-ZsGreen1-miR-193a-3p plasmid in HEK293 cells ( Lower ).

    Article Snippet: The slides were incubated with the primary polyclonal anti-GRB7 antibody (H70, Santa Cruz Biotechnology, Inc.) at a 1:50 dilution.

    Techniques: Expressing, Comparison, Binding Assay, Western Blot, Control, Transfection, Mutagenesis, Luciferase, Reporter Assay, Activity Assay, Plasmid Preparation

    MiR-193a-3p targets not only GRB7, but other key factors along MAPK/ERK signaling pathway in ovarian cancer cells. (A) The Venn diagram displays 77 common putative targets of miR-193a-3p predicted from the three algorithms, miRDB, miRanda and TargetScan Human 5.2, including three putative targets, ERBB4, KRAS and SOS2, involved in the MAPK/ERK signaling pathway. (B) Functional annotation analysis using the DAVID functional annotation tool ( http://david.abcc.ncifcrf.gov/ ) showing the main signaling pathways associated with these 78 common targets of miR-193a-3p . (C) Duolink proximity ligation assay (PLA) with fluorescence and confocal microscopy showing the interactions between GRB7 and SOS2 and between GRB7 and KRAS (red dots) by transient transfection of GRB7-expressing plasmid ( Upper ). Anti-Myc, anti-SOS2 and anti-KRAS were used to detect Myc-tagged GRB7 and endogenous SOS2 and KRAS. Transient transfection of HA-SOS2 also showing the interaction between SOS2 and KRAS (red dots) using PLA and anti-HA as well as anti-KRAS ( Lower ). Scale bar, 20 μm. n = 3 independent experiments. (D) Relative luciferase activity of luciferase reporters with wild-type ERBB4, SOS2 and KRAS 3'UTRs co-transfected with miR-193a-3p . (E) Western blot analysis showing that transient transfection of GFP/GRB7-expressing plasmid profoundly elevated ERK activity in HEK293, SKOV3 and OVCA433 cells. (F) Western blot analysis indicating that transient transfection of pmR-ZsGreen1-miR-193a-3p reduced the expression of GRB7, ERBB4, SOS2, KRAS and pERK1/2 in SKOV3 cells. Cell lysates of all the above cell transfectants were harvested 24 h after cell transfection.

    Journal: Theranostics

    Article Title: Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways

    doi: 10.7150/thno.22377

    Figure Lengend Snippet: MiR-193a-3p targets not only GRB7, but other key factors along MAPK/ERK signaling pathway in ovarian cancer cells. (A) The Venn diagram displays 77 common putative targets of miR-193a-3p predicted from the three algorithms, miRDB, miRanda and TargetScan Human 5.2, including three putative targets, ERBB4, KRAS and SOS2, involved in the MAPK/ERK signaling pathway. (B) Functional annotation analysis using the DAVID functional annotation tool ( http://david.abcc.ncifcrf.gov/ ) showing the main signaling pathways associated with these 78 common targets of miR-193a-3p . (C) Duolink proximity ligation assay (PLA) with fluorescence and confocal microscopy showing the interactions between GRB7 and SOS2 and between GRB7 and KRAS (red dots) by transient transfection of GRB7-expressing plasmid ( Upper ). Anti-Myc, anti-SOS2 and anti-KRAS were used to detect Myc-tagged GRB7 and endogenous SOS2 and KRAS. Transient transfection of HA-SOS2 also showing the interaction between SOS2 and KRAS (red dots) using PLA and anti-HA as well as anti-KRAS ( Lower ). Scale bar, 20 μm. n = 3 independent experiments. (D) Relative luciferase activity of luciferase reporters with wild-type ERBB4, SOS2 and KRAS 3'UTRs co-transfected with miR-193a-3p . (E) Western blot analysis showing that transient transfection of GFP/GRB7-expressing plasmid profoundly elevated ERK activity in HEK293, SKOV3 and OVCA433 cells. (F) Western blot analysis indicating that transient transfection of pmR-ZsGreen1-miR-193a-3p reduced the expression of GRB7, ERBB4, SOS2, KRAS and pERK1/2 in SKOV3 cells. Cell lysates of all the above cell transfectants were harvested 24 h after cell transfection.

    Article Snippet: The slides were incubated with the primary polyclonal anti-GRB7 antibody (H70, Santa Cruz Biotechnology, Inc.) at a 1:50 dilution.

    Techniques: Functional Assay, Protein-Protein interactions, Proximity Ligation Assay, Fluorescence, Confocal Microscopy, Transfection, Expressing, Plasmid Preparation, Luciferase, Activity Assay, Western Blot

    DNA methylation leads to downregulation of miR-193a-3p during the tumor development and progression of ovarian cancer cells. (A) qPCR analysis revealing a stepwise decrease in miR-193a-3p expression from Stages 1 to 4 and Grades 1 to 3 in ovarian cancers. (B) Representative photographs showing an inverse correlation between miR-193a-3p and GRB7 expression by ISH and IHC analyses for serous subtype ovarian cancer samples (early stage = Stage 1 and Grade 2, and advanced stage = Stage 2 and Grade 3) on a commercial ovarian cancer tissue array (OVC1021, Pantomics). Scale bar, 100 μm. (C) The expression of miR-193a-3p could be restored in ovarian cancer cells upon treatment with 5-Aza-dc (5 μM) for 4 days. (D) Verification of miR-193a-3p promoter methylation by MS-PCR in ovarian cancer cells upon 5-Aza-dC treatment. Treatment with 5-Aza-dC (5 μM) for 3-4 days. (E) Pyrosequencing analysis showing a progressive increase in DNA methylation with the tumor stage ( Upper ) and grade ( Lower ) of ovarian cancers.

    Journal: Theranostics

    Article Title: Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways

    doi: 10.7150/thno.22377

    Figure Lengend Snippet: DNA methylation leads to downregulation of miR-193a-3p during the tumor development and progression of ovarian cancer cells. (A) qPCR analysis revealing a stepwise decrease in miR-193a-3p expression from Stages 1 to 4 and Grades 1 to 3 in ovarian cancers. (B) Representative photographs showing an inverse correlation between miR-193a-3p and GRB7 expression by ISH and IHC analyses for serous subtype ovarian cancer samples (early stage = Stage 1 and Grade 2, and advanced stage = Stage 2 and Grade 3) on a commercial ovarian cancer tissue array (OVC1021, Pantomics). Scale bar, 100 μm. (C) The expression of miR-193a-3p could be restored in ovarian cancer cells upon treatment with 5-Aza-dc (5 μM) for 4 days. (D) Verification of miR-193a-3p promoter methylation by MS-PCR in ovarian cancer cells upon 5-Aza-dC treatment. Treatment with 5-Aza-dC (5 μM) for 3-4 days. (E) Pyrosequencing analysis showing a progressive increase in DNA methylation with the tumor stage ( Upper ) and grade ( Lower ) of ovarian cancers.

    Article Snippet: The slides were incubated with the primary polyclonal anti-GRB7 antibody (H70, Santa Cruz Biotechnology, Inc.) at a 1:50 dilution.

    Techniques: DNA Methylation Assay, Expressing, Methylation

    Clinico-pathological correlation of the expression of miR193a-3p in an ovarian cancer tissue array (OVC1021). The 4-fold and 3-fold cut-off points of miR-193a-3p and GRB7, respectively, were determined by ROC analysis.

    Journal: Theranostics

    Article Title: Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways

    doi: 10.7150/thno.22377

    Figure Lengend Snippet: Clinico-pathological correlation of the expression of miR193a-3p in an ovarian cancer tissue array (OVC1021). The 4-fold and 3-fold cut-off points of miR-193a-3p and GRB7, respectively, were determined by ROC analysis.

    Article Snippet: The slides were incubated with the primary polyclonal anti-GRB7 antibody (H70, Santa Cruz Biotechnology, Inc.) at a 1:50 dilution.

    Techniques: Expressing

    Re-introduction of miR-193a-3p suppresses cell proliferation, cell migration and invasion capacities of ovarian cancer cells. (A) qPCR and Western blot analyses of miR193a-3p and GRB7 expression in stable miR-193a-3p-expressing SKOV3 and OVCA433 cells. SNORD48 was used as an internal control. (B) XTT proliferation assay, (C) Transwell cell migration and invasion assays showing that stable re-expression of miR-193a-3p inhibited cell proliferation rates, cell migration and invasion abilities of SKOV3 and OVCA433 ovarian cancer cells.

    Journal: Theranostics

    Article Title: Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways

    doi: 10.7150/thno.22377

    Figure Lengend Snippet: Re-introduction of miR-193a-3p suppresses cell proliferation, cell migration and invasion capacities of ovarian cancer cells. (A) qPCR and Western blot analyses of miR193a-3p and GRB7 expression in stable miR-193a-3p-expressing SKOV3 and OVCA433 cells. SNORD48 was used as an internal control. (B) XTT proliferation assay, (C) Transwell cell migration and invasion assays showing that stable re-expression of miR-193a-3p inhibited cell proliferation rates, cell migration and invasion abilities of SKOV3 and OVCA433 ovarian cancer cells.

    Article Snippet: The slides were incubated with the primary polyclonal anti-GRB7 antibody (H70, Santa Cruz Biotechnology, Inc.) at a 1:50 dilution.

    Techniques: Migration, Western Blot, Expressing, Control, Proliferation Assay

    The functional role of GRB7 knockdown on cell proliferation, migration, invasion, and in vivo tumorigenicity. (A) Western blot analysis showing the levels of GRB7 knockdown (KD) and scrambled control (SCR) in SKOV3 cells. (B) XTT proliferation assay and (C) Transwell cell migration and invasion assays showing that knockdown of GRB7 inhibited cell proliferation, migration and invasion. (D) The 3-4-week-old female nude mice were i.p . injected with GRB7 knockdown or scrambled control SKOV3 cells and photographed on Day 60. Yellow arrows indicate the location of tumor nodules. The number of tumor nodules on the last day in each group are illustrated by bar charts (N=5).

    Journal: Theranostics

    Article Title: Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways

    doi: 10.7150/thno.22377

    Figure Lengend Snippet: The functional role of GRB7 knockdown on cell proliferation, migration, invasion, and in vivo tumorigenicity. (A) Western blot analysis showing the levels of GRB7 knockdown (KD) and scrambled control (SCR) in SKOV3 cells. (B) XTT proliferation assay and (C) Transwell cell migration and invasion assays showing that knockdown of GRB7 inhibited cell proliferation, migration and invasion. (D) The 3-4-week-old female nude mice were i.p . injected with GRB7 knockdown or scrambled control SKOV3 cells and photographed on Day 60. Yellow arrows indicate the location of tumor nodules. The number of tumor nodules on the last day in each group are illustrated by bar charts (N=5).

    Article Snippet: The slides were incubated with the primary polyclonal anti-GRB7 antibody (H70, Santa Cruz Biotechnology, Inc.) at a 1:50 dilution.

    Techniques: Functional Assay, Knockdown, Migration, In Vivo, Western Blot, Control, Proliferation Assay, Injection

    DOX-induced miR-193a-3p inhibits cell migration and invasion in vitro and results in complete inhibition of tumor growth. (A) qPCR and (B) Western blot analysis showing induction of the expression of GRB7 by 1 μg/ml doxycycline (Dox) in a time-dependent manner. SNORD48 was used as an internal control. (C) XTT cell proliferation assay showing the effect of inducible miR-193a-3p on SKOV3 cells. (D) Transwell cell migration/invasion assays demonstrating the suppressive effect of inducible miR-193a-3p on the migration and invasion capacities of SKOV3 ovarian cancer cells. The induction of miR-193a-3p for cell proliferation and cell migration/invasion using 1 μg/ml Dox. (E) Schematic overview of miR-193a-3p expression in mice and its effect on the suppression of ovarian tumor growth in the intraperitoneal cavity of nude mice. (F) The 3-4-week-old female mice received an intraperitoneal injection (i.p.) of 1x10 6 miR-193a-3p -inducible SKOV3 cells. When palpable tumors formed on Day 20, all mice were treated with or without 2 mg/mL Dox in the drinking water until they were sacrificed on Day 60. Representative photographs of tumor nodules collected after induction of miR-193a-3p on Day 60. The table shows that only 1 out of 4 mice had tumor nodules in the Dox-inducible miR-193a-3p group, while all mice (4 out of 4) developed tumor nodules in the control group. The number of tumor nodules on the last day is illustrated for each group by bar charts (N=4).

    Journal: Theranostics

    Article Title: Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways

    doi: 10.7150/thno.22377

    Figure Lengend Snippet: DOX-induced miR-193a-3p inhibits cell migration and invasion in vitro and results in complete inhibition of tumor growth. (A) qPCR and (B) Western blot analysis showing induction of the expression of GRB7 by 1 μg/ml doxycycline (Dox) in a time-dependent manner. SNORD48 was used as an internal control. (C) XTT cell proliferation assay showing the effect of inducible miR-193a-3p on SKOV3 cells. (D) Transwell cell migration/invasion assays demonstrating the suppressive effect of inducible miR-193a-3p on the migration and invasion capacities of SKOV3 ovarian cancer cells. The induction of miR-193a-3p for cell proliferation and cell migration/invasion using 1 μg/ml Dox. (E) Schematic overview of miR-193a-3p expression in mice and its effect on the suppression of ovarian tumor growth in the intraperitoneal cavity of nude mice. (F) The 3-4-week-old female mice received an intraperitoneal injection (i.p.) of 1x10 6 miR-193a-3p -inducible SKOV3 cells. When palpable tumors formed on Day 20, all mice were treated with or without 2 mg/mL Dox in the drinking water until they were sacrificed on Day 60. Representative photographs of tumor nodules collected after induction of miR-193a-3p on Day 60. The table shows that only 1 out of 4 mice had tumor nodules in the Dox-inducible miR-193a-3p group, while all mice (4 out of 4) developed tumor nodules in the control group. The number of tumor nodules on the last day is illustrated for each group by bar charts (N=4).

    Article Snippet: The slides were incubated with the primary polyclonal anti-GRB7 antibody (H70, Santa Cruz Biotechnology, Inc.) at a 1:50 dilution.

    Techniques: Migration, In Vitro, Inhibition, Western Blot, Expressing, Control, Proliferation Assay, Injection